Recruiting

Nitrous Oxide Concentrations in Blood, Exhaled Air, and Urine After Different Exposures

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Study Aim

This study aims to determine the time it takes for Nitrous Oxide to be detected in your blood after different levels of exposure.

What is being tested

Nitrous Oxide 99 %

+ Nitrous Oxide 99 %

+ Nitrous Oxide 99 %

Other
Who is being recruted

Criminal Behavior+1

+ Behavior

+ Dangerous Behavior

From 18 to 65 Years
See all eligibility criteria
How is the trial designed

Other Study

Interventional
Study Start: June 2026
See protocol details

Summary

Principal SponsorUniversity of Aarhus
Study ContactCharlotte U Andersen, Associate ProfessorMore contacts
Last updated: July 8, 2026
Sourced from a government-validated database.Claim as a partner

Study start date: June 1, 2026

Actual date on which the first participant was enrolled.

This study focuses on understanding how much nitrous oxide, a type of gas, ends up in the blood, exhaled breath, and urine after different types of exposure. It looks at three scenarios: breathing in nitrous oxide from cartridges in a car, eating whipped cream made with nitrous oxide, and being exposed to it through work as a midwife in a delivery room. The goal is to see how these different situations might lead to varying levels of nitrous oxide in the body. This research is important as it can help us understand more about how nitrous oxide affects the body in different circumstances and potentially lead to improved safety measures. During the study, the levels of nitrous oxide in the blood and exhaled breath are checked for up to an hour after exposure. For urine, the checks continue for up to two hours. The blood and urine samples are tested using gas chromatography and mass spectrometry, while the breath is tested using a special sensor. One of the main things the study wants to find out is how long after exposure nitrous oxide can still be detected in the blood.

Principal SponsorUniversity of Aarhus
Study ContactCharlotte U Andersen, Associate ProfessorMore contacts
Last updated: July 8, 2026
Sourced from a government-validated database.Claim as a partner

Protocol

This section provides details of the study plan, including how the study is designed and what the study is measuring.
Design Details

40 patients to be enrolled

Total number of participants that the clinical trial aims to recruit.

Other Study

Some studies explore topics that don't fall into a specific category. These might include innovative research, new technologies, or emerging healthcare areas.



Eligibility

Researchers look for people who fit a certain description, called eligibility criteria: person's general health condition or prior treatments.
Conditions
Criteria

Any sex

Biological sex of participants that are eligible to enroll.

From 18 to 65 Years

Range of ages for which participants are eligible to join.

Healthy volunteers allowed

If individuals who are healthy and do not have the condition being studied can participate.

Conditions

Pathology

Criminal BehaviorBehaviorDangerous BehaviorDriving Under the Influence

Criteria

For arm 1, 2, and 3: Inclusion Criteria: * Age 18-65 years * Able to understand Danish * Willing to use effective contraception for 24 hours after the end of exposure (oral contraceptives, contraceptive implant, transdermal patch, vaginal ring, or injectable contraceptives) * Written informed consent Exclusion Criteria: * Current or past abuse of drugs, nitrous oxide (N₂O), or alcohol * A psychiatric disorder requiring medical treatment, including severe depression * A diagnosis of epilepsy * Symptoms of peripheral neuropathy * Significant cardiac or pulmonary disease * Previous allergic or adverse reaction to N₂O * Diving within the past 2 days * Inability to refrain from diving for 2 days after the study * Occupational exposure to N₂O * Use of N₂O within the past 3 months * Treatment with methotrexate, benzodiazepines, opioids, or other centrally depressant drugs * Recent intraocular gas injection * Known impaired liver or kidney function * Pregnant or breastfeeding women (fertile women, defined as amenorrhea for less than 12 months, must have a negative hCG pregnancy test prior to receiving the study intervention)

Study Plan

Find out more about all the medication administered in this study, their detailed description and what they involve.
Treatment Groups
Study Objectives

4 intervention groups are designated in this study

This study does not include a placebo group 

Treatment Groups

Group I

Experimental
A person sits for 15 minutes after releasing 4 x 8.4 g of nitrous oxide into the cabin

Group II

Experimental
A person sits for 15 minutes after releasing 4 x 8.4 g of nitrous oxide into the cabin

Group III

Experimental
A person ingests 100 g of whipped cream with nitrous oxide as propellant

Group IV

Active Comparator
concentrations of nitrous oxide are measured after exposure through work as a midwife in a delivery room

Study Objectives

Primary Objectives

Study Centers

These are the hospitals, clinics, or research facilities where the trial is being conducted. You can find the location closest to you and its status.

This study has 1 location

Recruiting

Department of Forensic Medicine

Aarhus, DenmarkOpen Department of Forensic Medicine in Google Maps
Recruiting
One Study Center