Completed

Impact of Nuun Instant on Hydration Status in Active Men and Women

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What is being tested

Nuun Instant

+ Water

+ Nuun Electrolyte

Dietary SupplementOther
Who is being recruted

From 18 to 45 Years
+9 Eligibility Criteria
See all eligibility criteria
How is the trial designed

Basic Science Study

Placebo-Controlled
Interventional
Study Start: February 2021
See protocol details

Summary

Principal SponsorUniversity of Memphis
Last updated: January 28, 2026
Sourced from a government-validated database.Claim as a partner

Study start date: February 23, 2021

Actual date on which the first participant was enrolled.

Maintaining adequate hydration is essential to optimal health and athletic performance. When individuals exercise (in particular in a warm environment), they can lose excessive amounts of fluids through sweating, along with necessary electrolytes (e.g., sodium, potassium, chloride). With dehydration, individuals may feel sluggish and can experience impaired physical performance. Many attempts have been made to improve and measure the hydration status of active individuals. This typically involves the ingestion of fluids leading up to activity (typically plain water), as well as the ingestion of fluids during the activity itself (water, along with a diluted carbohydrate/electrolyte beverage). This approach seems to work well; however, some debate remains over what the best fluid is to consume, in particular related to the macronutrient type and the specific electrolyte mix. Related to the above, it is well-accepted that electrolyte replenishment is of importance, both during and following exercise-to aid in rehydration for subsequent bouts. Electrolytes (sodium in particular) have been used for decades to aid athlete hydration and this has led to the development of various sport drinks-which also include small amounts of carbohydrate (e.g., Gatorade, Powerade). However, one problem with moderate to high carbohydrate ingestion is that some individuals experience gastrointestinal (GI) upset following carbohydrate ingestion before and during an event, despite very good physical performance outcomes. Due to this GI upset, some individuals (in particular recreationally active individuals who are not competing at high levels) rely on water alone or a very low concentrated carbohydrate beverage, and seek a method to ingest the lost electrolytes as well. The solution to the above issues for many athletes is the use of powder "stick packs" that can be carried in bags and pockets, and simply dumped into a water bottle when needed, shaken, and consumed. They are easy to transport and provide the needed carbohydrate, as well as electrolytes to replace those that are lost through intense and/or long duration exercise. We recently completed a study of Nuun electrolyte tablets, which provide 2 grams of carbohydrate per serving and a combination of electrolytes, provided at a relatively low percentage of the Daily Value (DV). The DV tells us how much a particular nutrient in a serving of a food product contributes to a daily diet, based on a standard 2000 calorie diet. Nuun tablets contain: calcium (13mg, 1%DV), sodium (300mg, 13%DV), potassium (150mg, 3%DV), magnesium (25mg, 6%DV), and chloride (40mg, 2%DV). Our results indicated improved hydration (based on urine output and the calculated hydration index) when subjects consumed the Nuun tablets mixed into 1 liter of water, as compared to water alone. Nuun Instant is a new product that is similar to the Nuun tablets, but is formulated for those who may experience a significant electrolyte loss and is higher in carbohydrate as well. Specifically, the Nuun Instant product contains (per 11 gram serving) the following: 7 grams of carbohydrate, calcium (20mg, 2%DV), sodium (520mg, 23%DV), potassium (385mg, 8%DV), magnesium (8mg, 2%DV), chloride (250mg, 10%DV), and 100% of vitamin C (90mg) and B12 (2.4 mcg). Nuun Electrolytes is a new product in development that is similar to the Nuun tablets, but with an adjusted formula. Nuun Electrolytes consist of Citric Acid, Dextrose, Sodium Bicarbonate, Potassium Bicarbonate, Sodium Carbonate, Natural Flavors, Potassium Chloride, Malic Acid, Magnesium Carbonate, Calcium Carbonate, Stevia Leaf Extract, Beet Powder Color, Safflower Oil. Nuun electrolytes contains (per tablet) the following: 3 grams of carbohydrate, calcium (15 mg, 1%DV), sodium (200 mg, 9%DV), potassium (125mg, 3%DV), magnesium (15mg, 4%DV), and chloride (75mg, 3%DV). While Nuun Instant is commercially available and has received positive reviews from athletes, there have been no studies to date to evaluate the impact of this product or Nuun Electrolyte on hydration status. Therefore, the purpose of this study is to determine the impact of Nuun Instant powder and Nuun Electrolyte tablets on hydration status in young and active men and women. We will follow a similar approach as used by Maughan and colleagues (2016) to measure the fluid balance (the difference between the amount of water consumed and passed as urine) and beverage hydration index (the relative amount of urine passed after consumption of a drink compared to water), while also measuring plasma volume using the method of Dill and Costill (1974).

Official TitleImpact of Nuun Instant on Hydration Status in Active Men and Women
NCT04764565
Principal SponsorUniversity of Memphis
Last updated: January 28, 2026
Sourced from a government-validated database.Claim as a partner

Protocol

This section provides details of the study plan, including how the study is designed and what the study is measuring.
Design Details

20 patients to be enrolled

Total number of participants that the clinical trial aims to recruit.

Basic Science Study

Basic science studies help researchers understand how the body works or how a disease develops. They don't test treatments, but they build the foundation for future therapies.



Eligibility

Researchers look for people who fit a certain description, called eligibility criteria: person's general health condition or prior treatments.
Criteria

Any sex

Biological sex of participants that are eligible to enroll.

From 18 to 45 Years

Range of ages for which participants are eligible to join.

Healthy volunteers allowed

If individuals who are healthy and do not have the condition being studied can participate.

Criteria

6 inclusion criteria required to participate
body mass index (BMI) between 18-29.9 kg/m2

no consumption of alcohol-containing beverages within 48 hours of testing

no consumption of caffeine-containing beverages within 48 hours of testing

no strenuous exercise within 48 hours of testing

Show More Criteria

3 exclusion criteria prevent from participating
tobacco user

active infection or illness

pregnant

Study Plan

Find out more about all the medication administered in this study, their detailed description and what they involve.
Treatment Groups
Study Objectives

3 intervention groups are designated in this study

33.333% chance of being blinded to the placebo group

Treatment Groups

Group I

Experimental
2 servings of Nuun instant in 1 liter water

Group II

Placebo
1 liter of water

Group III

Experimental
2.1 servings of Nuun Electrolyte in 1 liter water

Study Objectives

Primary Objectives

Study Centers

These are the hospitals, clinics, or research facilities where the trial is being conducted. You can find the location closest to you and its status.

This study has 1 location

Suspended

Center for Nutraceutical and Dietary Supplement Reseach

Memphis, United StatesOpen Center for Nutraceutical and Dietary Supplement Reseach in Google Maps
CompletedOne Study Center