Effect of Hypoxia and Smoking on Oxidation of Proteins and Nucleic Acids in Human Volunteers
Data Collection
Collected from today forward - ProspectiveCase-Only
Examining characteristics of individuals with a disease in order to identify genetic or environmental factors contributing to the condition.Summary
Study start date: August 22, 1995
Actual date on which the first participant was enrolled.Stress such as high oxygen or inflammation can result in damage to proteins by processes such as oxidation or alternative regulation of signaling pathways by post-translational modification of proteins (e.g., phosphorylation). Delivery of oxygen in high concentrations to the lungs can result in damage, which is mediated in large part by reactive oxygen species. Inflammation can result in activation of intracellular signaling pathways. This study will evaluate modification of proteins and nucleic acids in bronchoalveolar lavage fluid, bronchial epithelial cells, and peripheral blood of individuals exposed to oxygen or who are smokers. In doing so, it will determine the effects of hyperoxia or inflammation on the lung.
Protocol
This section provides details of the study plan, including how the study is designed and what the study is measuring.77 patients to be enrolled
Total number of participants that the clinical trial aims to recruit.Case-only
Eligibility
Researchers look for people who fit a certain description, called eligibility criteria: person's general health condition or prior treatments.Any sex
Biological sex of participants that are eligible to enroll.From 18 to 65 Years
Range of ages for which participants are eligible to join.Healthy volunteers allowed
If individuals who are healthy and do not have the condition being studied can participate.Criteria
INCLUSION CRITERIA: History - good overall health without history of recent (within 3 months) acute disease; Physical examination within normal limits; Laboratory evaluation; including complete blood count (CBC), serum electrolyte determinations, clotting times, chest x-ray, pulmonary function testing, and an electrocardiogram (EKG) - within normal limits; Non-smokers defined as having never smoked or not smoked in the past 2 years; Smokers defined as moderate (1 pack per day for 3+ years) or heavy (1-2 packs for 10+ years); Subjects must be willing to make the time commitment necessary for the study. EXCLUSION CRITERIA: Any study subject who does not fulfill the criteria for eligibility. Individuals with a history of allergy or adverse reactions to atropine or any local anesthetic; Individuals testing positive for the human immunodeficiency virus or hepatitis virus; Individuals on chronic medications or currently receiving medications; Pregnant or lactating individuals, since the effects of hyperoxia on the fetus are unclear.
Study Plan
Find out more about all the medication administered in this study, their detailed description and what they involve.One single intervention group is designated in this study
This study does not include a placebo group
Treatment Groups
Study Objectives
Primary Objectives
Study Centers
These are the hospitals, clinics, or research facilities where the trial is being conducted. You can find the location closest to you and its status.This study has 1 location
National Institutes of Health Clinical Center, 9000 Rockville Pike
Bethesda, United StatesOpen National Institutes of Health Clinical Center, 9000 Rockville Pike in Google Maps