ALVAC-HIV candidate vaccines have induced HIV-specific CTL responses in more than half of recipients in some protocols. Depending on the HIV-1 gene products expressed by the particular ALVAC-HIV candidate vaccine, volunteers have generated anti-Envelope (vCP125, vCP205, and vCP300), anti-Gag (vCP205 and vCP300), and anti-Nef (vCP300) CTL activity. Although 3 to 4 immunizations with the different ALVAC-HIV experimental vaccines induce anti-HIV-1 neutralizing antibodies in a portion, often the majority, of volunteers, the geometric mean titers of these antibodies are modest, usually less than 50. This study will determine whether there is an increase in the anti-HIV antibody titers when GM-CSF is used as an adjuvant with ALVAC-HIV vCP205 and will also examine the kinetics and magnitude of the HIV-specific CTL response. In this randomized, placebo-controlled, double-blinded study volunteers receive ALVAC-HIV vCP205 at 10\^6.3 TCID50 or placebo and GM-CSF or placebo by intramuscular injection at Months 0, 1, 3, and 6 as follows: Group A: vCP205 plus GM-CSF placebo (10 volunteers) Group B: vCP205 plus 80 microg GM-CSF (10 volunteers) Group C: vCP205 plus 250 microg GM-CSF (10 volunteers) Group D: vcP205 placebo plus GM-CSF placebo (6 volunteers). \[AS PER AMENDMENT 04/30/99: Boosting with APL-400-047 HIV-1 gag/pol DNA is added for volunteers who have received all scheduled immunization in the original protocol. Volunteers in Groups A, B, and C will receive booster intramuscular injections of DNA vaccine at Months 0 and 1, those in Group D will receive DNA control (bupivacaine carrier alone) at Months 0 and 1\].
Inclusion Criteria Volunteers must have: * Negative ELISA for HIV within 8 weeks prior to immunization. * CD4 count of 400 cells/mm3 or higher. * Normal history and physical examination. * Viable EBV line prior to initial immunization. \[AS PER AMENDMENT 4/30/99: * Negative anti-dsDNA antibodies (for volunteers receiving booster vaccine).\] Exclusion Criteria Co-existing Condition: Volunteers with the following conditions or symptoms are excluded: * Medical or psychiatric condition or occupational responsibilities that preclude compliance with the protocol. * Recent suicidal ideation or psychosis. * Active syphilis. NOTE: * If the serology is documented to be a false positive or due to a remote (greater than 6 months) treated infection, the volunteer is eligible. * Active tuberculosis. NOTE: * Volunteers who have a positive PPD and a normal chest x-ray showing no evidence of TB and who do not require INH therapy are eligible. * Positive for hepatitis C antibody or hepatitis B surface antigen. * Allergy to eggs, neomycin, or thimerosal. \[AS PER AMENDMENT 4/30/99: * Hypersensitivity to bupivacaine or other amide-type anesthetics (e.g., lidocaine, mepivacaine) for volunteers receiving booster vaccine).\] Concurrent Medication: Excluded: Lithium or cimetidine. Volunteers with the following prior conditions are excluded: * History of immunodeficiency, chronic illness, or autoimmune disease. * History of cancer unless there has been surgical excision with reasonable assurance of cure. * History of suicide attempts or past psychosis. * History of anaphylaxis or other serious adverse reactions to vaccines. * History of serious allergic reaction to any substance requiring hospitalization or emergent care (e.g., Stevens-Johnson syndrome, bronchospasm, or hypotension). \[AS PER AMENDMENT 11/13/97: * History of cardiac disease or cardiac arrhythmias.\] Prior Medication: Excluded: * Live attenuated vaccines within 60 days of study. NOTE: * Medically indicated subunit or killed vaccines (e.g., influenza, pneumococcal) are not exclusionary, but should be given at least 2 weeks away from HIV immunizations. * Experimental agents within 30 days prior to study. * Blood products or immunoglobulin in the past 6 months. * HIV-1 vaccines or placebo as part of a previous HIV vaccine trial. * Immunosuppressive medications. Risk Behavior: Excluded: Volunteers with an identifiable higher-risk behavior for HIV infection (i.e., AVEG Risk Group C or D), including a history of injection drug use within 12 months prior to enrollment or higher-risk sexual behavior as defined by the AVEG.