Coffee and Oxidative Stress During Exercise in Healthy Humans

Study start date: March 1, 2012

Inclusion Criteria: Healthy Normal and overweight subjects (BMI 19 - 29), with lean mass below the 90th percentile value Coffee drinkers with an average consumption of 1-3 cups per day Having given informed consent VO2 max as a function of age and gender, not higher than the values below: Max oxygen uptake (VO2max, in ml/kg/min) in non athletes Age Males Females 20-29 49 41 30-39 47 39 40-49 45 37 Exclusion Criteria: Digestive (intestinal, gastric, hepatic or pancreatic), renal or metabolic disease, as determined by the medical (screening) visit and a blood chemistry analysis (glucose, cholesterol, LDL, HDL, triglycerides, aspartate transaminase (ASAT), Alanine transaminase (ALAT), C reactive Protein (CRP), creatinin) Subjects at cardio-vascular risk rated as intermediate or high (according to the Swiss Society of Cardiology, lipids group (www.agla.ch/p11-1-2.html), meaning those who show at least one of the following criteria: History of coronary disease / atherosclerosis Diabetes High level of risk (familial hypercholesterolemia, hyperlipoproteinemia type III, hypertension) Metabolic syndrome defined by the presence of at least 3 out of the following criteria : Abdominal circumference > 102 cm in men, > 88 cm in women Fasting triglycerides ≥ 1.7 mmol/l HDL-cholesterol < 1.0 mmol/l in men, < 1.3 mmol/l in women Systolic arterial blood pressure ≥ 130 mm Hg and/or diastolic arterial blood pressure ≥ 85 mm Hg Plasma glucose ≥ 6.1 mmol/l Anemia (Erythrocytes < 4.6 T/l (male) or < 4.2 T/l (women); Hemoglobin Hb < 13 g/dl (male) or Hb < 12 g/dl (women); Hematocrit Ht < 40% (male) or Ht < 37% (women); sera iron < 0.6 mg/l or plasma ferritin < 120 microg /l (male) or < 60 microg/l (non menopausal women) Troubles of hemostasis as determined by platelets number, prothrombin time and activated partial thromboplastin time Have had a gastrointestinal surgery, except appendicectomy Pregnancy History of food or medication allergy Have a regular consumption of medication (only those impacting oxidative stress biomarkers (eg. impacting the metabolism of lipids / LDL / VLDL), oral contraception excepted) Have taken antibiotic therapy within the last 3 months Smokers (more than 5 cigarettes per day) Having given blood within the last month, or willing to make a blood donation until one month following the end of the study Have a high alcohol consumption (more than 2 drinks/day) Taking more than 3 cups of coffee per day Consumption of illicit drugs Subject who cannot be expected to comply with the study procedures, including consuming the test products. Currently participating or having participated in another clinical trial during the last month prior to the beginning of this study.