A Phase I Study of Autologous, Activated CD8(+) Lymphocytes Expanded In Vitro and Infused With or Without Recombinant Interleukin-2 to Patients With AIDS or Severe ARC
Zidovudine
+ Aldesleukin
Infecciones Transmitidas por la Sangre+13
+ Enfermedades Urogenitales
+ Enfermedades Genitales
Estudio de Tratamiento
Resumen
CD8(+) cells are suppressor/killer lymphocyte cells that act to limit replication of viruses. It is hoped that the reinfusion of activated autologous CD8(+) cells into patients with AIDS will help to control opportunistic infections such as cytomegalovirus and toxoplasmosis (two of the leading causes of sickness and death in AIDS patients). This treatment may also stop the HIV virus from replicating (reproducing itself) in the AIDS patient. Further activation of these cells, once infused, may be necessary. It is hoped that IL-2 will stimulate the patient's immune system against the AIDS virus along with the activated CD8(+) cells. Thus, IL-2 will be given, and its effects studied. AMENDED: 09/28/90 The CD8 lymphocytes are grown in vitro for 21 days before infusion. Leukapheresis and infusion continue every 21 days for 3 infusions with the last infusion during week 16. During weeks 13 and 16, indium 111 radio labelled cells are injected to permit determination of the distribution and pharmacokinetics of the infused cells. At week 16, IL-2 is administered concurrently with Indium 111 labelled cells and the CD8+ lymphocytes - delivered by continuous infusion over 5 days following cell infusion. Patients are followed at the clinic 1 week prior to scheduled infusions, during infusion weeks and then every 2 weeks to week 21 and at week 27. Original design: Patients undergo leukapheresis every 2 weeks for a total of 6 times during the initial phase of the study. During this procedure, a catheter is placed in an arm vein, and the blood flows through a machine which separates the lymphocytes from the other blood components. The blood is then returned to the patient's body through the catheter. Less than 10 percent of the lymphocytes in the blood are removed during the process. The CD8(+) cells taken from the patient are cultured in the laboratory until they increase 10- to 1000-fold. These cells are then infused back into the patient from whom they were taken. The first two patients are admitted for 24 hours at the time of each infusion. In the absence of severe side effects, the subsequent four patients are infused at the clinic. Patients are admitted for 5 days for continuous infusion of IL-2 in week 13.
Protocolo
Esta sección proporciona detalles del plan del estudio, incluyendo cómo está diseñado y qué se está evaluando.Se reclutarán 6 pacientes
Número total de participantes que el ensayo clínico espera reclutar.Estudio de Tratamiento
Elegibilidad
Los investigadores buscan pacientes que cumplan ciertos criterios, conocidos como criterios de elegibilidad: estado general de salud o tratamientos previos.Cualquier sexo
Sexo biológico de los participantes elegibles para inscribirse.A partir de 18 años
Rango de edades de los participantes que pueden unirse al estudio.Voluntarios sanos no permitidos
Indica si personas sanas, sin la condición que se estudia, pueden participar.Condiciones
Patología
Criterios
Inclusion Criteria Concurrent Medication: Required: Zidovudine (AZT) during treatment and for 20 weeks after the last infusion unless medically contraindicated. Allowed: Aerosolized pentamidine for Pneumocystis carinii pneumonia (PCP) prophylaxis. Oral antibiotics for PCP prophylaxis if hematologically stable on that regimen for at least 30 days prior to study entry. Patients must have the following: Positive HIV antibody test by federally licensed ELISA. Positive HIV culture or plasma p24 antigen. CDC Group IV severe AIDS-related complex (ARC) or AIDS. Must have been on zidovudine (AZT) at least 6 weeks prior to infusion and agree to continue this medication during the study and for 20 weeks after the last infusion unless medically contraindicated. Allowed: Kaposi's sarcoma. Exclusion Criteria Co-existing Condition: AMENDED: Pulmonary diseases that require treatment. AMENDED: Significant central nervous system disease including AIDS dementia, psychiatric disabilities, or seizure disorders. AMENDED: Symptomatic HIV CNS infections or symptoms compatible with HIV encephalopathy. Original design: Patients with the following conditions or symptoms are excluded: Active bacterial or opportunistic infection that requires treatment. Neoplasms not specifically allowed, basal cell carcinoma of the skin, or in-situ carcinoma of the cervix. Clinically significant cardiac (= or > class II, New York Heart Association) or peripheral vascular disease that requires treatment. Hemorrhagic diathesis including hemophilia or active bleeding disorder. Concurrent Medication: Excluded: Antineoplastic therapy. Medication required for treatment of active cardiac disease. Cardiac glycosides. Antiarrhythmics. Antianginal agents. Anticoagulants. Thrombolytic agents. Vasodilators. Excluded within 30 days of study entry: Antiretroviral agents not specifically allowed. Corticosteroids. Acyclovir. Excluded within 60 days of study entry: Biological response modifiers. Patients with the following are excluded: Unable to give properly informed consent by reason of impaired mentation. Diseases and conditions specified elsewhere in the protocol. Required: Zidovudine (AZT) for at least 6 weeks prior to infusion. Risk Behavior: Excluded: Active substance abuse.
Centros del Estudio
Estos son los hospitales, clínicas o centros de investigación donde se lleva a cabo el estudio. Puedes encontrar la ubicación más cercana a ti y su estado de reclutamiento.Este estudio tiene una ubicación
Univ of Pittsburgh Med School
Pittsburgh, United StatesAbrir Univ of Pittsburgh Med School en Google Maps